Neonatal Cholestasis


Definition

Hepatobiliary dysfunction leading to pathologic jaundice in the neonatal period. Distinct from physiologic jaundice of the newborn. Cholestasis is the reduction in bile formation or flow which leads to elevated serum levels of direct/conjugated bilirubin and bile acids (cholic acid, chenodeoxycholic acid, etc…) instead of excretion into bile and elimination in feces.
Nelsons: "prolonged elevation of the serum levels of conjugated bilirubin beyond the first 14 days of life."

Cholestasis can be classified by origin:

Epidemiology

Clinical Manifestations

Evaluation

Patients with any of the following should be evaluated for cholestatic jaundice:


Red Flags for Neonatal Cholestasis
Red Flags Prompting Further Evaluation of Neonatal Cholestasis
Item of concern Why it is concerning
Consanguinity Increased risk of autosomal recessive disorders.
Neonatal cholestasis in the parents or siblings Cystic fibrosis, alpha-1 antitrypsin deficiency, progressive familial intrahepatic cholestasis, and Alagille syndrome are all genetic conditions causing neonatal cholestasis.
Family history of repeated fetal loss or early demise Suggestive of gestational alloimmune liver disease.
Spherocytosis and other hemolytic diseases Known to aggravate conjugated hyperbilirubinemia.
Prenatal ultrasonography findings Presence of choledochal cyst, cholelithiasis, bowel anomalies, or concern for a syndrome.
Maternal cholestasis of pregnancy May be seen in heterozygotes for PFIC gene mutations; may also suggest mitochondrial disorders.
Maternal acute fatty liver of pregnancy Associated with neonatal long-chain 3-hydroxyacyl-coenzyme A dehydrogenase (LCHAD) deficiency.
Maternal infections TORCH infections (Toxoplasmosis, “other”/syphilis, Rubella, CMV, HSV).
Gestational age Prematurity is a risk factor for neonatal hepatitis.
Small for gestational age (SGA) Increased risk of neonatal cholestasis and congenital infections.
Alloimmune hemolysis; G6PD deficiency; hydrops fetalis Increased risk of neonatal cholestasis.
Neonatal infection Urinary tract infection, sepsis-related cholestasis, CMV, HIV, syphilis, etc.
Newborn screen abnormalities Panhypopituitarism, galactosemia, fatty acid oxidation defects, cystic fibrosis.
Source of nutrition: breast milk, formula, parenteral nutrition (PN) Galactosemia, hereditary fructose intolerance, and PN-associated liver disease.
Appearance Dysmorphic features suggestive of syndromes such as Alagille’s (consider murmur).
Growth May indicate underlying genetic or metabolic disease.
Vision Septo-optic dysplasia and optic nerve hypoplasia, often associated with panhypopituitarism.
Hearing Associated with PFIC1, TJP2 and other cholestatic disorders.
Vomiting May indicate metabolic disease, bowel obstruction, or pyloric stenosis.
Stooling pattern Delayed stooling: cystic fibrosis, panhypopituitarism (check genitalia); diarrhea: infection or metabolic disease.
Stool color Acholic stools suggest biliary obstruction and cholestasis.
Urine characteristics: smell and color Dark urine suggests conjugated hyperbilirubinemia or metabolic disease.
Excessive bleeding May indicate coagulopathy or vitamin K deficiency.
Disposition: irritability, lethargy May reflect metabolic disease, sepsis, or panhypopituitarism.
History of abdominal surgery Necrotizing enterocolitis, intestinal atresia, or other surgical conditions affecting the biliary tree or intestine.
Neonatal exposure to fluconazole or micafungin Drugs that can cause cholestasis.


Tier 1

Tier 2 (Targeted based on findings/history)

Treatment


Note: Direct bilirubin reported using the diazo or van den bergh method includes: conjugated bilirubin and delta bilirubin (conjugated bilirubin that is covalently bound to albumin).

Prior definitions of cholestasis included direct >2mg/dL or 20% of total. This is now replaced with >1mg/dL


References:
NASPGHAN - CPG on neonatal cholestasis
Nelsons - Textbook Peds
https://www.naspghan.org/files/documents/pdfs/position-papers/Guideline_for_the_Evaluation_of_Cholestatic.23.pdf
https://enewsletter.cincinnatichildrens.org/physicians/gastroenterology/2019/06/new-test-expedites-the-diagnosis-of-biliary-atresia
https://pubmed.ncbi.nlm.nih.gov/29167395/

MMP7 Lab form